General Molecular Biology:

• Primer/construct design

• Cloning

• Gene block assembly

Protein Expression and Purification:

• E.coli, yeast, insect cell, mammalian cell types

• Protein yield optimization

• Purification at all scales

  • Low-scale

    • Including high-throughput, 384- or 96- well formats

  • Mid-scale

    • 0.5 - 10 L cultures

  • Manufacturing development-scale

    • Bioreactors up to 60 L

• Chromatography

  • FPLC/HPLC/UHPLC/FLASH

  • Immobilized metal affinity

  • Biological tags (GST/MBP/HA/FLAG/etc.)

  • Ion-exchange

  • Size-exclusion / gel filtration

Biophysical Characterization of Proteins and Ligands

• Thermofluor stability and buffer optimization

• Equilibrium denaturation

• Fluorescence anisotropy/polarization

• Isothermal calorimetry

• Differential-scanning calorimetry

• Prolyl-isomerization

• Bead-based immunoprecipitation/pulldown

• Surface plasmon resonance / bio-layer interferometry

• Mass spectrometry

• NMR

• Protein Crystallography

• In-vitro and cell-based assays

Additional techniques

• Enzyme engineering

• Bioconjugation

• Diffraction-limited fluorescence microscopy

• Immunohistochemistry/immunofluorescence

• NGS sample prep

• Molecular dynamics

• DOCK

• DMBA/TPA tumorigenesis in mice

• IP/ROS/tail vein immunizations in mice

• FACS/imaging cytometry

• Cell-based neutralization assays

• Mitochondrial integrity assays

• Automated liquid handlers

• High-performance computing

• Python

• DOCK / Autodock

• MOE

• ImageJ / FIJI

• Matlab

• R

• Chimera

• PyMOL

• AMBER

• VMD

• Empower

• ChemStation

• Analyst

• SCIEX OS

• PMI

• Chromeleon

• Microsoft, Apple, and Unix operating environments

• Microsoft Office Applications

• Adobe Creative Suite

• UNCLE

• QuikBooks

• SharePoint

• TeamViewer

• NoMachine

• Mendeley

• FileZilla

• Prezi

• HKL2000

• Phenix

• phaser

• LabView

• Origin

• AutoCAD

• Solidworks

• Maple

• Ideas

• Cyflogic

• Flowing Software

• SnapGene

• Serial Cloner

• Vector NTI (RIP)

• Lebedev, I., Nemajerova, A., Kornaj, M., Tong, M., Moll, U., Seeliger, M. (August, 2016). A novel in vitro CypD-mediated p53 aggregation assay suggests a model for mitochondrial permeability transition by chaperone systems. Journal of Molecular Biology.

• Feldman, H. C., Tong, M., Wang, L., Meza-Acevedo, R., Gobillot, T. A., Lebedev, I., … Maly, D. J. (May, 2016). Structural and Functional Analysis of the Allosteric Inhibition of IRE1α with ATP-Competitive Ligands. ACS Chemical Biology.

• Lebedev, I., Feldman, H. C., Georghiou, G., Maly, D. J., Seeliger, M. (2016). Src kinase in complex with a sulfonamide inhibitor. PDB:5J5S. http://www.rcsb.org/pdb/explore.do?structureId=5J5S

Fluent: English and Russian

Basic: German and Spanish